I
have often been asked what I consider to be the most impressive scientific
discovery of my own lifetime. It is a good question, capable of invoking endless
fascinating debate. In my view, though, one stroke of post-1966 genius stands
head and shoulders above all else. If I could – in my dreams – have been
responsible for just one piece of research, this would undoubtedly be it.
The
story began in Alameda County, California in 1983. A young post-doctoral
biochemist was driving late one night with his girlfriend (also a scientist)
when a promising idea occurred to him. His hypothesis, if proven, would bring
about the exact replication of a short strand of DNA, with all base-pairs
(genetic building blocks) in their correct sequence. Each replicate could then
itself be replicated, over and over, until the tiniest trace of DNA had, by
simple addition of raw materials at optimum temperatures, been amplified, with
total fidelity, to create a significant mass. This process, called the polymerase chain reaction (PCR), has
since revolutionized not only biochemistry, but also genetics, forensic science
and modern medicine.
The
name of this brilliant American is Dr Kary B. Mullis (Figure 78.1). He was
rewarded in 1993 with, inevitably, the Nobel Prize in Chemistry and has since
been inducted into the (US) National Inventors Hall of Fame.
Figure
78.1: An early picture of Dr Kary Mullis (1944-). In his 1996 book entitled Making
PCR, anthropologist Paul Rabinow echoed my own feelings: ‘Committees and science journalists like
the idea of associating a unique idea with a unique person, the lone genius.
PCR is thought by some to be an example of teamwork, but by others as the
genius of one who was smart enough to put things together which were present to
all, but overlooked. For Mullis, the light bulb went off, but for others it did
not. This is consistent with the idea, that the prepared (educated) mind who is
careful to observe and not overlook, is what separates the genius scientist
from his many also smart scientists. The proof is in the fact that the person
who has the light bulb go off never forgets the “Ah” experience, while the
others never had this photochemical reaction go off in their brains.’
Copyright
© 2012 Datos Freak
I
have used Dr Mullis’s PCR technique in a university forensics laboratory
(Figure 78.2). Extracting DNA from a single colony of bacteria, then copying it
to perfection millions of times over, was utterly compelling. When I discovered
that my theory was workable, I was spellbound, not to mention completely in awe
of Mullis’s logic. Without PCR, my project would have been impossible, and DNA
science in general would still be in its dark ages.
Figure
78.2: A treasured photograph taken after a crime scene analysis. My
PCR-inspired forensics dissertation can be viewed at http://biohorizons.oxfordjournals.org/content/3/2/166.full.pdf
and I like to think the broad thrust of it can be understood by anyone prepared
to make the effort.
Copyright
© 2008 Paul Spradbery
Imagine,
then, how I felt when, earlier this week, I received a personal invitation
(Figure 78.3) to the Royal Institution of Great Britain (Figures 78.4 &
78.5), in London, in order to meet the Nobel Laureate himself and hear the
story of his far-reaching innovation on the thirtieth anniversary of its
publication. The event, sponsored by drug development company Altermune
Technologies, takes place on Monday (October 6th), and promises to be uniquely memorable.
Figure
78.3: A welcome invitation, sent to biotechnology academics, entrepreneurs,
investors, journalists ... and yours truly
Copyright
© 2014 Altermune Technologies
Figure
78.4: The Royal Institution of Great Britain, on Albemarle Street, London, has,
for more than two hundred years, brought science to the British public and to the
rest of the scientific world.
Copyright © 2013 Astellas Pharma Europe Ltd
Figure 78.5: British inventor and electrical pioneer
Michael Faraday (1791-1867) presented the Christmas Lectures at the Royal
Institution. His world-changing discoveries were made in its basement.
Copyright expired
Copyright
© 2014 Paul Spradbery